Online Resource

NGS: Single-cell RNA-sequencing Analysis

Single-cell transcriptomics is a powerful tool to study the heterogeneity of the cellular transcriptome at single-cell levels. This course aims to equip participants with essential skills to process RNA-sequencing reads from single-cell transcriptomics experiment and to perform downstream analyses of the single-cell gene expression data. This course is designed for participants with some experience in R programming and running command-line programs. We will be using published single-cell RNA-sequencing datasets to perform quality control, data normalisation, cell clustering, differential expression and trajectory analyses.

Keywords: NGS, RStudio

Target audience: Researcher, Postgraduate student, Clinician, Bioinformatician, Postgraduate researcher

Resource type: Online Resource

Authors: Hamid, Fursham; Lozano Gonzalez, Karla


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