Here, we have made some of the Structural bioinformatics courses' materials available for you to access any time. The last edition of the Structural bioinformatics course ran in October 2025.The materials from Structural bioinformatics 2025 and previous editions provide a comprehensive guide to the commonly used methods and tools in structural bioinformatics to analyse and interpret experimentally determined and AI-predicted macromolecular structure data.Structural biology, determining the three-dimensional shapes of biomacromolecules and their complexes, can tell us a lot about how these molecules function and the roles they play within a cell. Data derived from structure determination experiments and Artificial Intelligence (AI)-assisted structure prediction enables life-science researchers to address a wide variety of questions.